To the alternate vial type (when necessary), again using a syringe, and make sure the final pre-dried recipient vial (from Glen Research or pre-dried by the user) is opened at the very last minute. For use of an expensive amidite on an Expedite synthesizer, you may prefer to use a vial with an internal conical shape that makes small sample handling easy and convenient and allows maximum delivery of your amidite solutions. V-vials from Wheaton or Reacti-vials from Pierce are available in 2, 3 or 5 mL size and with the 20-400 neck that is directly compatible with the Expedite amidite port. Finally a description of our products (powder or oil) is mentioned on the Certificate of Analysis that we provide with each product. Specific information about dissolution and special instructions are written on this Certificate, when applicable. So please do not forget to check this important information before using the product.
It is well over 20 years since the first amino-modifier prototypes were described on a modified base1 and at the 5′ terminus2. However, even after all these years, we have to acknowledge that there is still no ideal amino-modifier currently available commercially. Glen Research is now pleased to offer customers a mature version of the amino-modifier C6 phosphoramidite. 1.1. Aminolink and Microarrays/DNA chips Amino-modified oligos are popularly used for microarray manufacturing since DNA chip technology has become such an indispensable tool for life sciences.1135243-19-4 Molecular Weight 3,4 Fabrication is based on in situ synthesis on silicon chips or the more accessible approach of post-synthetic immobilization of oligos onto activated surfaces, predominantly glass slides.86347-15-1 InChIKey Companies and oligo synthesis houses use high-throughput processing to prepare libraries of tens of thousands of aminomodified oligos which are then spotted on surfaces to yield microarrays.PMID:28722955 The spotting process uses either piezo-based dispensers or the more popular pin-based spotters. Procedures have been optimized mainly in terms of immobilization chemistries, with the most popular methods relying on the reaction of amino-modified oligos with surfaces derivatized with moieties such as isothiocyanate or epoxy5 groups. The quality of the product as well as the yield of these amino-modified oligos is vitally important since re-synthesis on this scale is time-consuming and expensive. Clearly, for this kind of high-volume throughput, purification of all these oligos using conventional methods, like HPLC or PAGE, is out of the question – although highthroughput reverse phase (RP) purification in cartridges and plates has proved to be useful. 1.2. Cartridge Purification The most popular amino-modifier used today contains an amine protected by a trifluoroacetyl (TFA) group, e.g., Amino-Modifier C6-TFA (10-1916). This modifier has the advantages of being inexpensive and reliable. However, it has flaws in that it does not allow the use of trityl-on purification techniques or even an estimate of the coupling yield by the trityl cation measurement. Moreover, since the removal of a TFA protecting group happens simultaneously with the deprotection of an oligo, side reactions such as Michael addition of acrylonitrile (formed from elimination of cyanoethyl protecting groups) take place, reducing the yield of the aminated product (http:// glenresearch//ProductFiles/Technical/ TB_avoidaminealkylation.html). A reagent more suitable for preparation of thousands of amino-modified oligos contains an amine.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
