E of exosome, which was verified to become very convenient and trusted. We think that this system is hugely productive and economical and has terrific potential to be additional used for the selective separation of exosome. Funding: This function was supported by a National Study Foundation (NRF) grant funded by the Korean government, Ministry of Education and Science Technologies (NRF-2017M2A2A6A01071157, NRF-2018R1C1B6008799).JOURNAL OF EXTRACELLULAR VESICLESPF10.11=OWP3.Aqueous two-phase technique to isolate extracellular vesicles for prostate cancer diagnosis Hyunwoo Shina, Jiyoon Kima, Mee Young Kimb, Yong Hyun Parkb, Yong Goo Kimc, Ji Youl Leeb and Jaesung Parkd POSTECH, Pohang, 5-LOX Antagonist Compound Republic of Korea; bDepartment of Urology, Seoul St. Mary’s Hospital, The Catholic University of Korea, Seoul, Republic of Korea, Seoul, Republic of Korea; cDepartment of Laboratory Medicine, Mary’s Hospital, The Catholic University of Korea, Seoul, Republic of Korea, Seoul, Republic of Korea; dDepartment of Mechanical Engineering, POSTECH, Pohang, Republic of Korea, Pohang, Republic of KoreaaIntroduction: Analyzing extracellular vesicles (EVs) is definitely an desirable means in prostate cancer diagnosis. Nonetheless, existing solutions of EVs isolation have low efficiency, purity and lengthy approach time, which induce low diagnostic ability. To method the challenges, we adapt a two-phase technique to diagnose prostate cancer by isolating EVs from patients’ urine. Applying the twophase program, prostate hyperplasia (BPH) sufferers and prostate cancer (PCA) patients were diagnosed, plus the diagnostic ability was compared with conventional diagnostic procedures. Techniques: Forty-two prostate cancer (PCA) sufferers and 20 benign prostate hyperplasia (BPH) patients’ urine, plasma, saliva was collected and utilized for identifying EVs isolation capacity of aqueous two-phase system(ATPS) and for comparing diagnostic capacity of ATPS with traditional diagnosis. Final results: With an optimized ATPS, EVs were isolated with an efficiency of around 90 . In addition, the EV-isolation time was inside approximately 30 min, and the purity of EVs in ATPS was approximately two times greater than accomplished with a conventional techniques, ultracentrifugation and polymeric precipitation. Immediately after the ATPS isolated EVs from patients’ physique fluid, PCR and ELISA were utilized to detect EVs derived from prostate cancer cells. The expression levels of RNA and protein markers of prostate cancer were compared, plus the connection in between expression levels and clinical mGluR1 Biological Activity information was analysed. The results demonstrated that diagnostic potential determined by ATPS was improved than other traditional solutions (serum PSA and sediments). In addition, sensitivity improved by no less than 10 , and specificity was enhanced by at the least 20 compared to traditional solutions. Summary/conclusion: Premium quality and quantity of EVs may be obtained from patients’ physique fluid making use of ATPS. Utilizing the abundant sources, which include cancer-related proteins and genes, we are able to perform a diagnosis with higher specificity and sensitivity. Thus, ATPS offers a potent tool for extra precise and sensitive diagnosis.ISEV2019 ABSTRACT BOOKPF11: EV-Based Therapeutics II Chairs: Yasnouri Fujita; Xue Zou Place: Level three, Hall A 15:306:PF11.Therapeutic effect of plant sap-derived nanovesicles on cancer cells Kim Kimina, Yeon Ju Hunb, Yoo Hye Jua and Ruri LeeaaUniversity of brain education, Cheon-an, Republic of Korea; bUniversity of British Columbia, cheonan, Republic of KoreaIntroduction: Mo.
