Ure was constructed by using hASCs exosomes, overexpression/silencing microrna-19 hASCs exosomes, to observe the survival price of rats, inflammatory markers of liver IRAK4 Inhibitor list tissue and pathological modifications of liver tissues. Results: The expression levels of il-10, il-1, il-6 and TNF- were the lowest, and also the silent group was the highest in vitro cell experiments.The lymphocyte apoptosis was the lightest and the silent group was probably the most critical inside the expression of microRNA-19 exosomes. Active oxygen and P47phox change with inflammatory variables. Inside the animal experiment, the survival price of the overexpressing microRNA-19 hASCs exosomes group was the highest, the liver tissue pathology, active oxygen and P47phox were the lowest, whilst the silent group was the opposite.Summary/Conclusion: MicroRNA-19 in the hASCs exosomes can inhibit liver tissue inflammation on the liver failure rat model induced by D gal.The remedy mechanism of exosomes is additional explored, for the future clinical use of hASCs exosomes to provide theoretical basis for treatment of hepatic failure patients.PT08.17 = OWP3.Origin of extracellular vesicles released in the course of exhaustive exerciseISEV 2018 abstract bookPT09: EVs in Autoimmunity and Sepsis Chairs: Lola Fernandez Messina; Fabiana Geraci Location: Exhibit Hall 17:15-18:PT09.01= OWP1.Role of CD4 in therapeutic mesenchymal stem cell-derived vesicles for joint diseasesPT09.Rheumatoid aspect is detected on circulating extracellular vesicles inside a subpopulation of rheumatoid arthritis patients with a additional extreme disease phenotype Onno Arntz1; Bartijn Pieters1; Rogier Thurlings2; Peter van de Kraan1; Fons van de Loo1PT09.Anti-inflammatory activity of exosome-mimetic IL-6 Inhibitor manufacturer nanovesicles from mesenchymal stem cells in septic mice Kyong-Su Park1; Ganesh V. Shelke2; Kristina Svennerholm3; Elga Bandeira1; Cecilia L ser2; Su Chul Jang4; Rakesh Chandode5; Inta Gribonika5; Jan L vall1 University of Gothenburg, Gothenburg, Sweden; 2Krefting Study Centre, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden; three Anesthesiology and Intensive Care Medicine, Institute of Clinical Science, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; 4 Krefting Analysis Centre, Institute of Medicine, University of Gothenburg, Boston, USA; 5Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, SwedenExperimental Rheumatology, Radboudumc, Nijmegen, The Netherlands; Rheumatology, Radboudumc, Nijmegen, The NetherlandsBackground: Sepsis remains a source of higher mortality in hospitalized patients regardless of proper antibiotics approaches. Treatment with exosomes from mesenchymal stem cells (MSCs) is an evolving field in sepsis as a result of their immunosuppressive properties. However, exosomes are naturally made at low quantities, and also the isolation approach is demanding. Lately, artificially generated nanovesicles (NVs) from cells happen to be applied to several disease models to overcome the disadvantages of exosomes. The aim of this study to decide no matter whether MSCs-derived NVs can suppress neighborhood and systemic inflammation in septic mice, and to elucidate the mechanism involved. Procedures: NVs were produced from bone marrow-derived MSCs by the breakdown of cells by means of serial extrusions by means of filters. Isolated NVs were analysed by transmission electron microscopy. Mice (C57BL/6) had been intraperitoneally injected with E. coli-derived outer membrane vesicles (OMVs) to establish sepsis, then.