Rdiomyocytes. Moreover, activation in the a1-AR can decrease myocardial TNF-a
Rdiomyocytes. Furthermore, activation of the a1-AR can lower myocardial TNF-a expression, maybe by means of activating ERK-c-Fos signalling and inhibiting NF-jB signalling, and enhance cardiac dysfunction in endotoxaemia. These findings define particular signalling molecular events that mediate the inhibitory effect of NE on LPS-induced TNF-a production in cardio2013 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley Sons Ltd and Foundation for Cellular and Molecular Medicine.myocytes, and might present potentially beneficial therapeutic targets for the remedy of myocardial depression in the course of sepsis.Foundation (S2011020005408) and fundamental Study Funds for the Central Universities (21611403).AcknowledgementsThis study was supported by grants in the National All-natural Science Foundation of China (81170222 and 30971191), the Guangdong Natural ScienceConflicts of interestThe authors confirm that you can find no conflicts of interest.
Int. J. Mol. Sci. 2014, 15, 1003-1013; doi:ten.3390ijmsOPEN ACCESSInternational Journal ofMolecular SciencesISSN 1422-0067 mdpijournalijms ArticleLactoferrin Directly Scavenges Hydroxyl Radicals and Undergoes Oxidative Self-Degradation: A Achievable Role in Protection against Oxidative DNA DamageYuki Ogasawara 1,, Megumi Imase two, Hirotsugu Oda 3, Hiroyuki Wakabayashi three and Kazuyuki IshiiDepartment of Analytical Biochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan Department of Hygienic Chemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan; E-Mails: 7tp-al.bl_u.u_lilb.bearezweb.ne.jp (M.I.); ishiimy-pharm.ac.jp (K.I.) Food Science Technology Institute, Morinaga Milk Business Co., Ltd., 5-1-83 Higashihara, Zama, Kanagawa 252-8583, Japan; E-Mails: h-odamorinagamilk.co.jp (H.O.); h_wakabymorinagamilk.co.jp (H.W.) Author to whom correspondence should be addressed; E-Mail: yomy-pharm.ac.jp; Tel.81-424-95-8652. Received: 19 November 2013; in revised form: 24 December 2013 Accepted: 9 January 2014 Published: 14 MAP3K5/ASK1 manufacturer JanuaryAbstract: Within this study, we examined the protective effect of lactoferrin against DNA damage induced by numerous hydroxyl radical generation systems. Lactoferrin (LF) was examined with regard to its possible function as a scavenger against radical oxygen species using bovine milk LF. Native LF, iron-saturated LF (holo-LF), and apolactoferrin (apo-LF) efficiently suppressed strand breaks in plasmid DNA as a consequence of hydroxyl radicals produced by the Fenton reaction. Also, both native LF and holo-LF EZH2 custom synthesis clearly protected calf thymus DNA from fragmentation because of ultraviolet irradiation within the presence of H2O2. We also demonstrated a protective impact of all 3 LF molecules against 8-hydroxydeoxyguanosine (8-OHdG) formation in calf thymus DNA following ultraviolet (UV) irradiation with H2O2. Our results clearly indicate that native LF has reactive oxygen species-scavenging capability, independent of its nature as a masking component for transient metals. We also demonstrated that the protective impact of LF against oxidative DNA damage is as a result of degradation of LF itself, which can be much more susceptible to degradation than other bovine milk proteins.Int. J. Mol. Sci. 2014, 15 Keywords: lactoferrin; bovine milk; DNA harm; hydroxyl radical; UV irradiationAbbreviations: LF, lactoferrin; EDTA, ethylenediaminetetraacetic acid; ROS, reactive oxygen species; 8-OHdG, 8-hydroxydeoxyguanosine; iron-saturated lactoferrin, holo-LF; a.
