Timothy R. Fennell, Robert M. Lust,* Jared M. Brown, and Christopher J. Wingard*,*Department of Physiology, Brody College of Medicine, East Carolina University, Greenville, North Carolina 27834; Department of Pharmacology Toxicology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27834; and Department of RTI International, Analysis Triangle Park, North Carolina1 Authors contributed equally. whom correspondence should be addressed at Department of Physiology, Brody College of Medicine at East Carolina University, 600 Moye Blvd, Brody 6N98, Greenville, NC 27834. Fax: (252) 744-3460. E-mail: [email protected] ToReceived July 25, 2013; accepted January 9,The possible uses of engineered C60 fullerene (C60 ) have expanded in current decades to incorporate industrial and biomedical applications. Based on clinical findings related with particulate matter exposure and our data with multi-walled carbon nanotubes, we hypothesized that ischemia/reperfusion (I/R) injury and pharmacological responses in isolated coronary arteries would rely upon the route of exposure and gender in rats instilled with C60 .IFN-gamma Protein Biological Activity Male and female Sprague Dawley rats had been made use of to test this hypothesis by surgical induction of cardiac I/R injury in situ 24 h soon after intratracheal (IT) or intravenous (IV) instillation of 28 g of C60 formulated in polyvinylpyrrolidone (PVP) or PVP car.SB-216 manufacturer Serum was collected for quantification of different cytokines.PMID:23724934 Coronary artery segments were isolated for assessment of vasoactive pharmacology by way of wire myography. Both IV and IT exposure to C60 resulted in expansion of myocardial infarction in male and female rats following I/R injury. Serum-collected post-I/R showed elevated concentrations of interleukin-6 and monocyte chemotactic protein-1 in male rats exposed to IV C60 . Coronary arteries isolated from male rats exposed to IT C60 demonstrated augmented vasocontraction in response to endothelin-1 that was attenuated with Indomethacin. IV C60 exposure resulted in impaired acetylcholine relaxation in male rats and IT C60 exposure resulted in depressed vasorelaxation in response to sodium nitroprusside in female rats. Determined by these data, we conclude that IT and IV exposure to C60 results in distinctive cardiovascular consequences that may possibly favor heightened coronary resistance and myocardial susceptibility to I/R injury. Crucial words: fullerene; nanotoxicology; myocardial infarction; cytokines; acetylcholine; endothelin-1.The heart may well be susceptible to infarction in response to ischemia/reperfusion (I/R) injury following pulmonary exposure to several kinds of nanosized particles ( 100 nm). WeDisclaimer: The authors are responsible for the conclusions described in this post, which may not represent those drawn by the National Institute of Environmental Wellness Sciences, East Carolina University or RTI International.have previously reported that post-I/R myocardial infarction worsens inside a dose- and time-dependent manner following intratracheal (IT) instillation of multi-walled carbon nanotubes (Urankar et al., 2012), cerium oxide nanoparticles (Wingard et al., 2010), or ultrafine particulate matter (Cozzi et al., 2006). Cardiovascular detriments connected with ultrafine particulate matter could result from pulmonary inflammation, oxidative strain, or direct particle effects following translocation (Campen et al., 2012; Utell et al., 2002). Exposure to nanosized particles can result in systemic release of interleukin-6 (IL.
